FluoS Loop-Mediated Amplification Kit

Product Description

This kit is supplied with BstL DNA Polymerase, thermostable Reverse Transcriptase, and the 5×buffer, which contains Mg2+, dNTP. A fluorescent dye is also supplied to enable real-time fluorescence measurement.

Product components

Usage

For DNA or RNA isothermal amplification using Probe or fluorescent dye.

Transportation And Storage Conditions

Transported with ice bags, stored at -20℃. Avoid repeated freezing and thawing, the product is valid for 12 months.

Protocol

1 Using Probe 1.1 Thaw buffer to be used at room temperature. Vortex briefly or invert tubes several times to mix thoroughly. Centrifuge to collect material and place on ice.

1.2. Prepare reaction mix as described below

10×Primer Mix: FIP/BIP 16μM，LoopF/B 4 μM，F3/B3 2 μM. Probe concentration may be optimized according to the specific situation.

1.3. Reaction program

Collect fluorescence signal.

2 Using fluorescent dye

1.1 Thaw buffer to be used at room temperature. Vortex briefly or invert tubes several times to mix thoroughly. Centrifuge to collect material and place on ice.

1.2. Prepare reaction mix as described below.