EasyVis Loop-Mediated Amplification Kit
Description
Product Information
Product Description
This kit is supplied with BstL DNA Polymerase, thermostable Reverse Transcriptase, and the 5×buffer, which contains Mg2+, dNTP, and visible dye. This kit provides a fast, clear visual detection of amplification, in which a negative reaction is indicated in the red and a positive reaction is indicated by a change to yellow.
Product components
Usage
For DNA or RNA isothermal amplification
Transportation and Storage Conditions
Transported with ice bags, stored at -20℃. Avoid repeated freezing and thawing, the product is valid for 12 months.
10×Primer Mix: FIP/BIP 16μM，LoopF/B 4 μM， F3/B3 2 μM
3. Incubate at 65°C for 30 – 45 minutes. Return reactions to 65℃ for an additional 10 minutes if the color is not yellow.
4. Examine color by eye, positive reactions will have turned yellow while negative controls should remain red.
Note:
1. Make primer, DNA/RNA stock in molecular biology grade H2O rather than TE or other buffers.
2. The reaction temperature can be optimized between 62°C and 68°C according to the primer conditions.
Protocol
- Thaw buffer to be used at room temperature and place on ice. Vortex briefly or invert tubes several times to mix thoroughly. Centrifuge to collect material and place on ice.
- Prepare reaction mix as described below.
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