This product is the Pyrophosphatase Inorganic recombinantly expressed in E. coli. After binding to magnesium ions, it catalyzes the hydrolysis of inorganic pyrophosphate to produce phosphate, and avoids its inhibition of the reaction system, thereby improving the yield of in vitro transcription of RNA.
Inorganic (yeast) (0.1
Optimal reaction temperature: 25℃. Definition of active unit: 1 active unit is defined as the amount of enzyme needed to hydrolyze inorganic pyrophosphate and produce 1 μmol of phosphate in 1min under standard reaction conditions (standard reaction conditions: in 10 min at 25℃, 0.5mL reaction system containing 100 mM Tris HCl, pH7.2, 2mM Mgcl 2 and 2mM inorganic pyrophosphate).
Purity ≥ 95%, residual host cell DNA ≤ 100pg/mg, residual host cell protein ≤ 50ppm, residual endotoxin ≤10EU/mg, no residual RNase, endonuclease, exonuclease or protease, germ-free, pathogen-free.
Effectively increasing the RNA yield of in vitro transcription.
Product TEST Picture
|EN-1009||Pyrophosphatase Inorganic (yeast)||10U, 100U|