Description
Product Introduction
This product is the RNase Ⅲ recombinantly expressed in E. coli. This specific exonuclease is capable of cutting double -stranded RNA (dsRNA) and generating 12-35bp dsRNA fragments with protruding 5′-PO4, 3′-OH, and 3’ termini.
Product Composition
| Item No | Composition | Storage Temperature (℃) |
Product ID/Specification | |
|---|---|---|---|---|
| EN-1007S (200U) |
||||
| EN-1007-I | RNase III (2U/μL) | -20 | 0.5mL | 5mL |
| EN-1007-Ⅱ | 10x Reaction Buffer | -20 | 1.5mL | 15mL |
| EN-1007-Ⅲ | 10x EDTA | -20 | 1mL | 10mL |
| EN-1007-Ⅳ | 10x MnCl 2 | -20 | 1mL | 1mL |
1x Reaction buffer contains 500mM Tris -HCl(pH7.5)500mM NaCl and 10mM DTT.
Product Properties
Optimal reaction temperature: 37℃. Definition of active unit: 1 active unit is defined as the amount of enzyme needed to degrade 1μg of dsRNA into siRNA in 20min at 37℃ in a 50μL reaction system.
Quality Control
Purity ≥ 95%, residual host cell DNA ≤ 100pg/mg, residual host cell protein ≤ 50ppm, residual endotoxin ≤10EU/mg, no residual RNase, endonuclease, exonuclease or protease, germ-free, pathogen-free.
Product Features
Efficient production of siRNA; accurate interference with target gene expression.
Product Information
| Item No | Product | Specification |
|---|---|---|
| EN-1007 | RNase Ⅲ | 200U, 2000U |
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