This product is the RNase Ⅲ recombinantly expressed in E. coli. This specific exonuclease is capable of cutting double -stranded RNA (dsRNA) and generating 12-35bp dsRNA fragments with protruding 5′-PO4, 3′-OH, and 3’ termini.
|EN-1007-I||RNase III (2U/μL)||-20||0.5mL||5mL|
|EN-1007-Ⅱ||10x Reaction Buffer||-20||1.5mL||15mL|
|EN-1007-Ⅳ||10x MnCl 2||-20||1mL||1mL|
1x Reaction buffer contains 500mM Tris -HCl(pH7.5)500mM NaCl and 10mM DTT.
Optimal reaction temperature: 37℃. Definition of active unit: 1 active unit is defined as the amount of enzyme needed to degrade 1μg of dsRNA into siRNA in 20min at 37℃ in a 50μL reaction system.
Purity ≥ 95%, residual host cell DNA ≤ 100pg/mg, residual host cell protein ≤ 50ppm, residual endotoxin ≤10EU/mg, no residual RNase, endonuclease, exonuclease or protease, germ-free, pathogen-free.
Efficient production of siRNA; accurate interference with target gene expression.
|EN-1007||RNase Ⅲ||200U, 2000U|