100 µg of protein a purified antibody at 1 mg/ml in 10 mm pbs, ph 7.2, containing 0.05% nan3
Shelf life:
1 year from date of receipt under proper storage conditions; aliquot to avoid multiple freeze thaw cycles
Host species:
Mouse
Isotype:
Igg2b
Clone:
His.h8
Immunogen:
6xhis synthetic peptide conjugated to klh
Specificity:
recognizes the epitope of n-terminal, c-terminal, or internal 6xhis-tags
Sensitivity:
1-2 ng of his-tagged fusion proteins (immunoblot/ecl)
Application:
Immunoblot (1:1000 to 1:3000; incubate 1 hr at room temp), immunostaining (1:500 to 1:2000) elisa, dot blot or immunoprecipitation (user to determine working dilution)
