Gentaur

Anti-Calmodulin Binding Peptide | Gentaur

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SKU:: 100-RCBP-45A-Z-GEN
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Description
Additional Information

Description

Anti-Calmodulin Binding Peptide | 100-RCBP-45A-Z

biological source

rabbit

Quality Level

100

antibody form

culture supernatant

antibody product type

primary antibodies

clone

C16T, monoclonal

species reactivity

vertebrates

technique(s)

western blot: suitable

isotype

IgG

UniProt accession no.

P62158

shipped in

dry ice

General description

Calmodulin Binding Protein (CBP) epitope tags are found in several E. coli expression vectors. Calmodulin (CaM), a small 17 kDa ubiquitous Ca2+ binding protein, acts as an intracellular Ca2+ receptor and transduces Ca2+ transients, usually in response to growth factors. It mediates the activity of a number of Ca2+ regulating enzymes, including protein kinases, phosphatases, nitric oxide synthases, and phosphodiesterase. Calmodulin is the principal intracellular calcium receptor. It is an acidic protein of 148 residues, and upon binding Ca2+, it in turn binds to short peptide sequences of many target proteins. Such interaction is thought to induce a conformational change on the target, resulting in its activation. Targets include the constitutively expressed nitric oxide synthases, the CaM kinases, and PP2B/Calcineurin.

Specificity

Recombinant proteins containing the calmodulin binding protein epitope tag.

Immunogen

Epitope: CBP epitope

KLH-conjugated, synthetic peptide corresponding to the Calmodulin Binding Protein (CBP) epitope tag found in several E. coli expression vectors.

Application

Research Category

Epitope Tags & General Use

Research Sub Category

Epitope Tags

This Anti-Calmodulin Binding Protein Epitope Tag Antibody, clone C16T is validated for use in WB for the detection of

Calmodulin Binding Protein Epitope Tag.

Quality

Evaluated by Western Blot on lysates from transformed E. coli.

Western Blot Analysis: 1:2,000 dilution of this antibody detected a recombinant protein containing the calmodulin binding protein epitope tag in lysates from transformed E. coli.

Target description

The molecular weight is related to the tagged protein.

Linkage

Replaces: 05-932

Physical form

Cultured supernatant with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at -20ºC from date of receipt.

Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
Samples containing the calmodulin binding protein epitope tag.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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