Product Introduction
This product is the mRNA Cap-2′-O-Methyltransferase of vaccinia virus recombinantly expressed in E. coli. With the RNA of 7-methylguanosine cap (Cap0) as the substrate, it specifically transfers the methyl of methylation donator SAM to the Cap0 structure in RNA to form the Cap1 structure, which can enhance the translation efficiency of mRNA and avoid the innate immune response in certain cells.
Product Composition
| Item No | Composition | Storage Temperature(℃) |
Product ID/Specification | |
|---|---|---|---|---|
| EN-1003S (2KU) | EN-1003L (20KU) | |||
| EN-1003-I | mRNA Cap-2′-OMethyltransferase(50U/μL) | -20 | 40μL | 400μL |
| EN-1003-Ⅱ | Capping Buffer | -20 | 100μL | 1000μL |
| EN-1003-Ⅲ | S-adENosylmethionine (SAM 32mM) |
-20 | 100μL | 1000μL |
1x Capping Buffer contains: 0.5M Tris -HCl(pH8.0), 50mM KCl, 10mM MgCl2 and 10mM DTT.
Product Properties
Optimal reaction temperature: 37℃. Definition of active unit: 1 active unit is defined as the amount of enzyme needed to incorporate 10 pmol of (α 32P)GTP into 80nt transcript within 1h at 37℃.
Quality Control
Purity ≥ 95%, residual host cell DNA ≤ 100pg/mg, residual host cell protein ≤ 50ppm, residual endotoxin ≤10EU/mg, no residual RNase, endonuclease, exonuclease or protease, germ-free, pathogen-free.
Product Features
In vitro capping can avoid in vivo immune response.
Product Information
| Item No | Product | Specification |
|---|---|---|
| EN-1003 | mRNA Cap-2′-OMethyltransferase | 2KU, 20KU |
