This product is the Vaccinia Capping Enzyme recombinantly expressed in E. coli. Vaccinia Capping Enzyme can add the 7-methylguanosine cap (Cap0) to the 5’ terminus of mRNA. This structure can improve the stability of mRNA, and is indispensable to subsequent transport and translation.
Enzyme (10 U/μL))
1x Capping Buffer contains: 0.5M Tris -HCl(pH8.0),50mM KCl,10mM MgCl2 and 10mM DTT.
Optimal reaction temperature: 37℃. Definition of active unit: 1 active unit is defined as the amount of enzyme needed to incorporate 10 pmol of (α 32P) GTP into 80nt transcript within 1h at 37℃.
Purity ≥ 95%, residual host cell DNA ≤ 100pg/mg, residual host cell protein ≤ 50ppm, residual endotoxin ≤10EU/mg, no residual RNase, endonuclease, exonuclease or protease, germ-free, pathogen-free.
Capping rate as high as 95%; the 5′-Cap structure effectively promoting the in vivo expression of mRNA.
|EN-1002||Vaccinia Capping Enzyme||400U, 4000U|