Vaccinia Capping Enzyme

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Product Introduction

This product is the Vaccinia Capping Enzyme recombinantly expressed in E. coli. Vaccinia Capping Enzyme can add the 7-methylguanosine cap (Cap0) to the 5’ terminus of mRNA. This structure can improve the stability of mRNA, and is indispensable to subsequent transport and translation.

Product Composition

Item No Composition Storage
Product ID/Specification  
EN-1002-I Vaccinia Capping
Enzyme (10 U/μL))
-20 40μL 400μL
EN-1002-Ⅱ Capping Buffer -20 100μL 1000μL
EN-1002-Ⅲ S-adENosylmethionine
(SAM 32mM)
-20 100μL 1000μL
EN-1002-Ⅳ GTP -20 50μL 500μL

1x Capping Buffer contains: 0.5M Tris -HCl(pH8.0),50mM KCl,10mM MgCl2 and 10mM DTT.

Product Properties

Optimal reaction temperature: 37℃. Definition of active unit: 1 active unit is defined as the amount of enzyme needed to incorporate 10 pmol of (α 32P) GTP into 80nt transcript within 1h at 37℃.

Quality Control

Purity ≥ 95%, residual host cell DNA ≤ 100pg/mg, residual host cell protein ≤ 50ppm, residual endotoxin ≤10EU/mg, no residual RNase, endonuclease, exonuclease or protease, germ-free, pathogen-free.

Product Features

Capping rate as high as 95%; the 5′-Cap structure effectively promoting the in vivo expression of mRNA.

Product Information

Item No Product Specification
EN-1002 Vaccinia Capping Enzyme 400U, 4000U
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