Vaccinia Capping Enzyme
- SKU:
- EN-1002
Description
Product Introduction
This product is the Vaccinia Capping Enzyme recombinantly expressed in E. coli. Vaccinia Capping Enzyme can add the 7-methylguanosine cap (Cap0) to the 5’ terminus of mRNA. This structure can improve the stability of mRNA, and is indispensable to subsequent transport and translation.
Product Composition
| Item No | Composition | Storage Temperature(℃) |
Product ID/Specification | |
|---|---|---|---|---|
| EN- 1002s(400U) |
EN- 1002L(4000U) |
|||
| EN-1002-I | Vaccinia Capping Enzyme (10 U/μL)) |
-20 | 40μL | 400μL |
| EN-1002-Ⅱ | Capping Buffer | -20 | 100μL | 1000μL |
| EN-1002-Ⅲ | S-adENosylmethionine (SAM 32mM) |
-20 | 100μL | 1000μL |
| EN-1002-Ⅳ | GTP | -20 | 50μL | 500μL |
1x Capping Buffer contains: 0.5M Tris -HCl(pH8.0),50mM KCl,10mM MgCl2 and 10mM DTT.
Product Properties
Optimal reaction temperature: 37℃. Definition of active unit: 1 active unit is defined as the amount of enzyme needed to incorporate 10 pmol of (α 32P) GTP into 80nt transcript within 1h at 37℃.
Quality Control
Purity ≥ 95%, residual host cell DNA ≤ 100pg/mg, residual host cell protein ≤ 50ppm, residual endotoxin ≤10EU/mg, no residual RNase, endonuclease, exonuclease or protease, germ-free, pathogen-free.
Product Features
Capping rate as high as 95%; the 5′-Cap structure effectively promoting the in vivo expression of mRNA.
Product Information
| Item No | Product | Specification |
|---|---|---|
| EN-1002 | Vaccinia Capping Enzyme | 400U, 4000U |
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