ATP-dependent T4 RNA Ligase

(No reviews yet) Write a Review
Frequently bought together:


Product Introduction

This product is the ATP-dependent T4RNA Ligase I recombinantly expressed in E. coli. It can catalyze oligonucleotide, single -stranded RNA and DNA intermolecular/ intramolecular 5’-PO4 and 3’-OH to form phosphodiester bond.

Product Composition

Item No Composition Storage
Product ID/Specification  
EN-1008-I T4 RNA Ligase
-20 0.1mL 1mL
EN-1008-Ⅱ Reaction Buffer -20 1.5mL 15mL
EN-1008-Ⅲ AdENosine-5′-Triphosphate (ATP 10mM) -20 0.2mL 2mL
EN-1008-Ⅳ PEG 8000 -20 1mL 10mL

1x Reaction buffer contains 50mM Tris -HCL (pH7.5), 10mM MgCL2 and 1mM DTT.

Product Properties

Optimal reaction temperature: 37℃. Definition of active unit: 1 active unit is defined as the amount of enzyme needed to convert 1nM 5’-[ 32P]rA16 into anti-phosphoric acid form in 30min at 37℃.

Quality Control

Purity ≥ 95%, residual host cell DNA ≤ 100pg/mg, residual host cell protein ≤ 50ppm, residual endotoxin ≤10EU/mg, no residual RNase, endonuclease, exonuclease or protease, germ-free, pathogen-free.

Product Usage

  • RNA 3′ terminus labelling (using cytidine-3′,5′-[ a- 32P] diphosphate)
  • Connecting RNAs
  • Synthesizing oligoribonucleotide and oligodeoxyribonucleotide
  • Specific modification of tRNA
  • Connecting oligodeoxyribonucleotide to single-stranded cDNA to realize 5’RACE (rapid amplification of cDNA termini)
  • Site-specific generation of multiplex PCR primer.

Product Information

Item NO Product Specification
EN-1008 T4RNA Ligase 1KU, 10KU
View AllClose