ATP-dependent T4 RNA Ligase
- SKU:
- EN-1008
Description
Product Introduction
This product is the ATP-dependent T4RNA Ligase I recombinantly expressed in E. coli. It can catalyze oligonucleotide, single -stranded RNA and DNA intermolecular/ intramolecular 5’-PO4 and 3’-OH to form phosphodiester bond.
Product Composition
| Item No | Composition | Storage Temperature(℃) |
Product ID/Specification | |
|---|---|---|---|---|
| EN-1008S (1KU) |
EN-1008L (10KU) |
|||
| EN-1008-I | T4 RNA Ligase (10U/μL)) |
-20 | 0.1mL | 1mL |
| EN-1008-Ⅱ | Reaction Buffer | -20 | 1.5mL | 15mL |
| EN-1008-Ⅲ | AdENosine-5′-Triphosphate (ATP 10mM) | -20 | 0.2mL | 2mL |
| EN-1008-Ⅳ | PEG 8000 | -20 | 1mL | 10mL |
1x Reaction buffer contains 50mM Tris -HCL (pH7.5), 10mM MgCL2 and 1mM DTT.
Product Properties
Optimal reaction temperature: 37℃. Definition of active unit: 1 active unit is defined as the amount of enzyme needed to convert 1nM 5’-[ 32P]rA16 into anti-phosphoric acid form in 30min at 37℃.
Quality Control
Purity ≥ 95%, residual host cell DNA ≤ 100pg/mg, residual host cell protein ≤ 50ppm, residual endotoxin ≤10EU/mg, no residual RNase, endonuclease, exonuclease or protease, germ-free, pathogen-free.
Product Usage
- RNA 3′ terminus labelling (using cytidine-3′,5′-[ a- 32P] diphosphate)
- Connecting RNAs
- Synthesizing oligoribonucleotide and oligodeoxyribonucleotide
- Specific modification of tRNA
- Connecting oligodeoxyribonucleotide to single-stranded cDNA to realize 5’RACE (rapid amplification of cDNA termini)
- Site-specific generation of multiplex PCR primer.
Product Information
| Item NO | Product | Specification |
|---|---|---|
| EN-1008 | T4RNA Ligase | 1KU, 10KU |
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