Phage T7 RNA Polymerase
This product is the phage T7 RNA Polymerase derived from recombinant expression in E. coli. It can specifically recognize the T7 promoter sequence and synthesize RNA complementary to downstream single-stranded DNA of the promoter using the single or double-stranded DNA containing the sequence of T7 promoter as the template and NTP as the substrate.
1x Buffer contains: 40 mM Tris HCl(pH7.9), 6mM MgCl 2, 1mM DTT and 2mM spermidine.
Optimal reaction temperature: 37℃. Definition of active unit: 1 active unit is defined as the amount of enzyme needed to incorporate 1 nmol of [ 3H] GMP into acid-insoluble precipitate within 1h under the conditions of 37℃ and pH8.
Purity ≥ 95%, residual host cell DNA ≤ 100pg/mg, residual host cell protein ≤ 50ppm, residual endotoxin ≤10EU/mg, no residual RNase, endonuclease, exonuclease or protease, germ-free, pathogen-free.
Excellent RNA output rate; homogeneous transcript length; transcript length up to 10K.
TEST RESULT PICTURE
|EN-1001||T7 RNA Polymerase||5KU, 50KU|