Poly(A) Polymerase

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Product Introduction

This product is the Poly(A) Polymerase recombinantly expressed in E. coli. It catalyzes with ATP as the substrate, and adds adenyl acid to the 3’- hydroxyl terminus of RNA to form the Poly A tail structure in the form of AMP.

Product Composition

Item No Composition Storage
Product ID/Specification  
EN-1004-I Poly(A) Polymerase
-20 40μL 400μL
EN-1004-Ⅱ Reaction Buffer -20 1.5mL 15mL
EN-1004-Ⅲ AdENosine-5’-
Triphosphate (ATP10mM)
-20 200μL 2000μL

1x Reaction Buffer contains 0.5M Tris -HCl(pH8.0), 50mM KCl and 10mM MgCl2.

Product Properties

Optimal reaction temperature: 37℃. Definition of active unit: 1 active unit is defined as the amount of enzyme needed to incorporate 1 nmol of AMP into RNA within 10min at 37℃ in a 20μL reaction system.

Quality Control

Purity ≥ 95%, residual host cell DNA ≤ 100pg/mg, residual host cell protein ≤ 50ppm, residual endotoxin ≤10EU/mg, no residual RNase, endonuclease, exonuclease or protease, germ-free, pathogen-free.

Product Features

Efficient mRNA capping: adding 200nt polyA tail to 10μg of RNA in 10min (5U enzyme for capping). Homogenous tailing length: controlling polyA tail length by adjusting reaction time or enzyme dosage.

Product TEST Picture

Product Information

Item No Product Specification
EN-1004 Poly(A) Polymerase 100U, 1000U