Description
Product Introduction
This product is the Poly(A) Polymerase recombinantly expressed in E. coli. It catalyzes with ATP as the substrate, and adds adenyl acid to the 3’- hydroxyl terminus of RNA to form the Poly A tail structure in the form of AMP.
Product Composition
| Item No | Composition | Storage Temperature(℃) |
Product ID/Specification | |
|---|---|---|---|---|
| EN- 1004S(100U) |
EN-1004L (1000U) |
|||
| EN-1004-I | Poly(A) Polymerase (50U/μL) |
-20 | 40μL | 400μL |
| EN-1004-Ⅱ | Reaction Buffer | -20 | 1.5mL | 15mL |
| EN-1004-Ⅲ | AdENosine-5’- Triphosphate (ATP10mM) |
-20 | 200μL | 2000μL |
1x Reaction Buffer contains 0.5M Tris -HCl(pH8.0), 50mM KCl and 10mM MgCl2.
Product Properties
Optimal reaction temperature: 37℃. Definition of active unit: 1 active unit is defined as the amount of enzyme needed to incorporate 1 nmol of AMP into RNA within 10min at 37℃ in a 20μL reaction system.
Quality Control
Purity ≥ 95%, residual host cell DNA ≤ 100pg/mg, residual host cell protein ≤ 50ppm, residual endotoxin ≤10EU/mg, no residual RNase, endonuclease, exonuclease or protease, germ-free, pathogen-free.
Product Features
Efficient mRNA capping: adding 200nt polyA tail to 10μg of RNA in 10min (5U enzyme for capping). Homogenous tailing length: controlling polyA tail length by adjusting reaction time or enzyme dosage.
Product TEST Picture
Product Information
| Item No | Product | Specification |
|---|---|---|
| EN-1004 | Poly(A) Polymerase | 100U, 1000U |
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