This product is the Alkaline Phosphatase recombinantly expressed in E. coli. It is capable of non-specific catalysis of the dephosphorylation of the phosphomonoester bond of DNA and RNA 5’ and 3’ termini, as well as catalysis of the dephosphorylation of NTPs and dNTPs.
1x Reaction buffer contains 50mM Bis -Tris-Propane-HCL, pH6, 1 mM MgCL2 and 0.1mM ZnCl 2.
Optimal reaction temperature: 37℃. Definition of active unit: 1 active unit is defined as the amount of enzyme needed to dephosphorylate 1μg of pUC19 plasmid vector in 30m at 37℃ (dephosphorylation is defined as ＞95% inhibitory action on re-cyclization in the self-linking reaction, and measured through transformation into E. coli.)
Purity ≥ 95%, residual host cell DNA ≤ 100pg/mg, residual host cell protein ≤ 50ppm, residual endotoxin ≤10EU/mg, no residual RNase, endonuclease, exonuclease or protease, germ-free, pathogen-free.
Dephosphorylation of DNA and RNA 5’ and 3’ termini. Can be used for DNA dephosphorylation before terminus -labelling by T4 polynucleotide kinase. Used for treatment of dNTPs in PCR reactions prior to sequencing or SNP analysis. Reducing the immunogenicity of mRNA in mammals by dephosphorylating mRNA. Avoiding re -cyclization during the cloning process by dephosphorylating DNA cloning vector. No BSA system, less heat source contamination