EcoRI 100KU, 5mL, 12.5mL

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Product Introduction

This product is the EcoRI capable of recognizing the G^AATTC site recombinantly expressed in E. coli. EcoRI is widely used in fields including gene mapping and cloning. It can cut DNA rapidly, to achieve gene linearization of high efficiency.

Product Composition

Item No Composition Storage
Temperature (℃)
Product ID/Specification  
      EN-1012S(10KU) EN-1012L (100KU)
EN-1012-I EcoRI (20 U/μL) -20 0.5mL 5mL
EN-1012-Ⅱ 10x Buffer -20 1.25mL 12.5mL

1x Buffer contains: 100 mM Tris-HCl, pH7.5, 50mM NaCl, 10mM MgCl 2 and 0.025% Triton X-100.

Product Properties

Optimal reaction temperature: 37℃. Definition of active unit: 1 active unit is defined as the amount of enzyme needed to degrade 1μgλ of DNA within 1h at 37℃ in a 50μL reaction system.

Quality Control

Purity ≥ 95%, residual host cell DNA ≤ 100pg/mg, residual host cell protein ≤ 50ppm, residual endotoxin ≤10EU/mg, no residual RNase, endonuclease, exonuclease or protease, germ-free, pathogen-free.

Product Features

High enzyme activity; rapid completion of cleavage reaction. Low non-specific endonuclease activity, to ensure “scalpel-like” precise cutting. No BSA system, less heat source contamination.

Product InformationSearch:

Item No Product Specification
EN-1012 EcoR I 10KU, 100KU
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