EcoRI 100KU, 5mL, 12.5mL
- SKU:
- EN-1012
Description
Product Introduction
This product is the EcoRI capable of recognizing the G^AATTC site recombinantly expressed in E. coli. EcoRI is widely used in fields including gene mapping and cloning. It can cut DNA rapidly, to achieve gene linearization of high efficiency.
Product Composition
| Item No | Composition | Storage Temperature (℃) |
Product ID/Specification | |
|---|---|---|---|---|
| EN-1012S(10KU) | EN-1012L (100KU) | |||
| EN-1012-I | EcoRI (20 U/μL) | -20 | 0.5mL | 5mL |
| EN-1012-Ⅱ | 10x Buffer | -20 | 1.25mL | 12.5mL |
1x Buffer contains: 100 mM Tris-HCl, pH7.5, 50mM NaCl, 10mM MgCl 2 and 0.025% Triton X-100.
Product Properties
Optimal reaction temperature: 37℃. Definition of active unit: 1 active unit is defined as the amount of enzyme needed to degrade 1μgλ of DNA within 1h at 37℃ in a 50μL reaction system.
Quality Control
Purity ≥ 95%, residual host cell DNA ≤ 100pg/mg, residual host cell protein ≤ 50ppm, residual endotoxin ≤10EU/mg, no residual RNase, endonuclease, exonuclease or protease, germ-free, pathogen-free.
Product Features
High enzyme activity; rapid completion of cleavage reaction. Low non-specific endonuclease activity, to ensure “scalpel-like” precise cutting. No BSA system, less heat source contamination.
Product InformationSearch:
| Item No | Product | Specification |
|---|---|---|
| EN-1012 | EcoR I | 10KU, 100KU |