This product is the EcoRI capable of recognizing the G^AATTC site recombinantly expressed in E. coli. EcoRI is widely used in fields including gene mapping and cloning. It can cut DNA rapidly, to achieve gene linearization of high efficiency.
|EN-1012-I||EcoRI (20 U/μL)||-20||0.5mL||5mL|
1x Buffer contains: 100 mM Tris-HCl, pH7.5, 50mM NaCl, 10mM MgCl 2 and 0.025% Triton X-100.
Optimal reaction temperature: 37℃. Definition of active unit: 1 active unit is defined as the amount of enzyme needed to degrade 1μgλ of DNA within 1h at 37℃ in a 50μL reaction system.
Purity ≥ 95%, residual host cell DNA ≤ 100pg/mg, residual host cell protein ≤ 50ppm, residual endotoxin ≤10EU/mg, no residual RNase, endonuclease, exonuclease or protease, germ-free, pathogen-free.
High enzyme activity; rapid completion of cleavage reaction. Low non-specific endonuclease activity, to ensure “scalpel-like” precise cutting. No BSA system, less heat source contamination.
|EN-1012||EcoR I||10KU, 100KU|